Studies on the Formation of Heinz Bodies. II. The Nature and Significance of Heinz Bodies

Phenyldiimide, hemoglobin, and Heinz bodies.

Phenylhydrazine is oxidized stoichiometrically by two equivalents of ferricyanide to produce phenyldiimide and ferrocyanide. This reaction permits accurate determination of the absorption maxima and molar absorption coefficients of phenyldiimide in aqueous solution. These values are identical or extremely similar to those of phenyldiimide produced by decarboxylation of phenylazoformic acid. Kno...

Heinz bodies interfere with automated reticulocyte counts.

Correspondence: Ignacio Español, M.D., Laboratoris Folguera, c/ Aribau 212-216, entl 3a, 08006 Barcelona, Spain. Phone: international +34-93-4143481 – Fax: international +34-932021341. was low: 20 mg/dL. A very high reticulocyte count was detected by two different automated counters (Figure 1), but only one reported a staining error. A crisis of hemolytic anemia or an error in the automated ret...

Effect of Heinz bodies on red cell deformability.

Decreased deformability of acetyldamaged cells, in an all-or-none phenylhydrazine-treated erythrocytes, fashion. In mixtures, separation of unmeasured by in vitro filtration experitreated cells from Heinz body-containments, was found to parallel Heinz ing erythrocytes could be accombody formation. The decreased deplished by filtration. There may be a formability seemed unrelated to merelationsh...

The response to Heinz bodies in the rat.

Observations on Heinz bodies in normal and splenectomized rabbits.